Chicken IL-8 ELISA Kit | IL-8 elisa kit
Chicken IL-8 (Interleukin 8) ELISA Kit
Gene Names
IL8; CXCL8; AMCF-I
Reactivity
Chicken
Synonyms
IL-8; N/A; Chicken IL-8 (Interleukin 8) ELISA Kit; IL-8 elisa kit
Reactivity
Chicken
Specificity
This kit recognizes Chicken IL-23 in samples. No significant cross-reactivity or interference between Chicken IL-23 and analogues was observed.
Sequence Length
103
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
18.75pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Chicken IL-23 were tested 20 times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Chicken IL-23 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for IL-8 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken IL-23 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken IL-23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Chicken IL-23. You can calculate the concentration of Chicken IL-23 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken IL-23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Chicken IL-23. You can calculate the concentration of Chicken IL-23 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,633 Da
NCBI Official Full Name
interleukin-8
NCBI Official Symbol
IL8
NCBI Official Synonym Symbols
CXCL8; AMCF-I
NCBI Protein Information
interleukin-8; C-X-C motif chemokine 8; IL-8; alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; chemokine (C-X-C motif) ligand 8
UniProt Protein Name
Interleukin-8
UniProt Gene Name
CXCL8
UniProt Synonym Gene Names
IL8; IL-8; AMCF-I
UniProt Entry Name
IL8_PIG
