Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human LUM. No significant cross-reactivity or interference between human LUM and analogues was observed.
Sequence Length
338
Samples
Serum, urine, cell culture supernates
Assay Type
Quantitative Sandwich
Detection Range
0.625 ng/ml-40 ng/ml
Sensitivity
< 0.156 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for LUM elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LUM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LUM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LUM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LUM bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,429 Da
NCBI Official Full Name
lumican
NCBI Official Synonym Full Names
lumican
NCBI Official Symbol
LUM
NCBI Official Synonym Symbols
LDC; SLRR2D
NCBI Protein Information
lumican; KSPG lumican; lumican proteoglycan; keratan sulfate proteoglycan lumican
UniProt Protein Name
Lumican
UniProt Gene Name
LUM
UniProt Synonym Gene Names
LDC; SLRR2D; KSPG lumican
UniProt Entry Name
LUM_HUMAN