Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit | sFLt-1 elisa kit

Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit

Cat. Num. AAA16281

• Gene Names: Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• Reactivity: Rat
• Synonyms: Soluble Fms-like tyrosine kinase Receptor 1; N/A; Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit; sFLt-1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of sFLTR-1. No significant cross-reactivity or interference between sFLTR-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLTR-1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for sFLt-1 elisa kit

Principle of the Assay: sFLTR-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sFLTR-1 antibody and an sFLTR-1-HRP conjugate. The assay sample and buffer are incubated together with sFLTR-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLTR-1 concentration since sFLTR-1 from samples and sFLTR-1-HRP conjugate compete for the anti-sFLTR-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLTR-1 from the sample, fewer sites are left to bind sFLTR-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLTR-1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This sFLTR-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sFLTR-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 2809071
• NCBI GeneID: 14254
• Molecular Weight: 149,876 Da
• NCBI Official Full Name: sFlt-1
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 1
• NCBI Official Symbol: Flt1
• NCBI Official Synonym Symbols: Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• NCBI Protein Information: vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: Flt1
• UniProt Synonym Gene Names: Emrk2; Flt; Vegfr1; VEGFR-1; FLT-1
• UniProt Entry Name: VGFR1_MOUSE