Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat Ang1-7. No significant cross-reactivity or interference between rat Ang1-7 and analogues was observed.
Sequence Length
497
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
18.75 pg/ml-1200 pg/ml
Sensitivity
<4.68 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for Ang1-7 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Ang1-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ang1-7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Ang1-7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ang1-7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,456 Da
NCBI Official Full Name
angiopoietin-1 isoform 2
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
ANGPT1
NCBI Official Synonym Symbols
AGP1; AGPT; ANG1
NCBI Protein Information
angiopoietin-1; ANG-1
UniProt Protein Name
Angiopoietin-1
UniProt Gene Name
ANGPT1
UniProt Synonym Gene Names
KIAA0003; ANG-1
UniProt Entry Name
ANGP1_HUMAN