Mouse Angiotensin (1-7) ELISA Kit | ANG (1-7) elisa kit

Mouse Angiotensin (1-7) ELISA Kit

Cat. Num. AAA16655

• Reactivity: Mouse
• Synonyms: Angiotensin (1-7); N/A; Mouse Angiotensin (1-7) ELISA Kit; ANG (1-7) elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ang1~7. No significant cross-reactivity or interference between Ang1~7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Ang1~7 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ANG (1-7) elisa kit

Principle of the Assay: Ang1~7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Ang1~7 antibody and an Ang1~7-HRP conjugate. The assay sample and buffer are incubated together with Ang1~7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ang1~7 concentration since Ang1~7 from samples and Ang1~7-HRP conjugate compete for the anti-Ang1~7 antibody binding site. Since the number of sites is limited, as more sites are occupied by Ang1~7 from the sample, fewer sites are left to bind Ang1~7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ang1~7 concentration in each sample is interpolated from this standard curve.

Intended Uses: This Ang1~7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Ang1~7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for ANG (1-7) elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 74765469
• NCBI Official Full Name: Angiotensin, partial
• UniProt Protein Name: Angiotensin
• UniProt Entry Name: ANGT_THETS