Rat matrix metallopeptidase 7 (matrilysin, uterine) ELISA Kit | MMP7 elisa kit
Rat matrix metalloproteinase 7, MMP-7 ELISA Kit
Gene Names
Mmp7; MPMM
Reactivity
Rat
Synonyms
matrix metallopeptidase 7 (matrilysin, uterine); N/A; Rat matrix metalloproteinase 7, MMP-7 ELISA Kit; Rat matrix metalloproteinase 7; MMP-7 ELISA kit; MMP-7; MPSL1; PUMP-1; matrin; matrix metalloproteinase 7; matrix metalloproteinase 7 (matrilysin; uterine) ; uterine matrilysin; matrix metallopeptidase 7 (matrilysin; uterine); MMP7 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat MMP-7. No significant cross-reactivity or interference between rat MMP-7 and analogues was observed.
Sequence Length
267
Samples
Serum, plasma and tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of rat MMP-7 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for MMP7 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,885 Da
NCBI Official Full Name
matrilysin
NCBI Official Synonym Full Names
matrix metallopeptidase 7
NCBI Official Symbol
Mmp7
NCBI Official Synonym Symbols
MPMM
NCBI Protein Information
matrilysin; MMP-7; matrin; pump-1 protease; uterine metalloproteinase; matrix metalloproteinase-7; Matrix metalloproteinase 7 (matrilysin)
UniProt Protein Name
Matrilysin
UniProt Gene Name
Mmp7
UniProt Synonym Gene Names
Mmp-7; MMP-7
UniProt Entry Name
MMP7_RAT
