Mouse terminal complement complex C5b-9, TCC C5b-9 ELISA Kit | TCC C5b9 elisa kit
Mouse terminal complement complex C5b-9, TCC C5b-9 ELISA Kit
Reactivity
Mouse
Synonyms
terminal complement complex C5b-9, TCC C5b-9; N/A; Mouse terminal complement complex C5b-9, TCC C5b-9 ELISA Kit; Mouse terminal complement complex C5b-9; TCC C5b-9 ELISA Kit; terminal complement complex C5b-9; TCC C5b-9; TCC C5b9 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TCC C5b-9. No significant cross-reactivity or interference between mouse TCC C5b-9 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
< 0.195 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for TCC C5b9 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TCC C5b-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TCC C5b-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TCC C5b-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TCC C5b-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.