Canine Matrix metalloproteinase 9 ELISA Kit | MMP-9 elisa kit

Canine Matrix metalloproteinase 9 ELISA Kit

Cat. Num. AAA16774

• Gene Names: MMP9; GELB; CLG4B; MMP-9; MANDP2
• Reactivity: Canine
• Synonyms: Matrix metalloproteinase 9; Canine Matrix metalloproteinase 9 ELISA Kit; MMP-9 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMP-9. No significant cross-reactivity or interference between MMP-9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-9 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MMP-9 elisa kit

Principle of the Assay: MMP-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MMP-9 antibody and an MMP-9-HRP conjugate. The assay sample and buffer are incubated together with MMP-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-9 concentration since MMP-9 from samples and MMP-9-HRP conjugate compete for the anti-MMP-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-9 from the sample, fewer sites are left to bind MMP-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-9 concentration in each sample is interpolated from this standard curve.

Intended Uses: This MMP-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MMP-9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for MMP-9 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 74272287
• NCBI GeneID: 4318
• NCBI Accession #: NP_004985.2
• NCBI GenBank Nucleotide #: NM_004994.2
• Molecular Weight: 78,458 Da
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• NCBI Official Symbol: MMP9
• NCBI Official Synonym Symbols: GELB; CLG4B; MMP-9; MANDP2
• NCBI Protein Information: matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• UniProt Protein Name: Matrix metalloproteinase-9
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: CLG4B; MMP-9; GELB
• UniProt Entry Name: MMP9_HUMAN