Human Desmoplakin ELISA Kit | DSP elisa kit
Human Desmoplakin ELISA Kit
Reactivity
Human
Synonyms
Desmoplakin; N/A; Human Desmoplakin ELISA Kit; DSP elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for DSP elisa kit
Intended Uses: This DES ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DES.
Principle of the Assay: DES ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DES. Standards or samples are then added to the microtiter plate wells and DES if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DES present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DES are added to each well to "sandwich" the DES immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DES and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DES concentration in each sample is interpolated from this standard curve.
Principle of the Assay: DES ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DES. Standards or samples are then added to the microtiter plate wells and DES if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DES present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DES are added to each well to "sandwich" the DES immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DES and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DES concentration in each sample is interpolated from this standard curve.
Product Categories/Family for DSP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,196 Da
NCBI Official Full Name
desmoplakin
NCBI Official Symbol
desmoplakin
NCBI Protein Information
desmoplakin
UniProt Protein Name
Desmoplakin
UniProt Gene Name
desmoplakin
UniProt Entry Name
Q7T9S0_GVAO
