Mouse bone morphogenetic protein 4 ELISA Kit | BMP4 elisa kit

Mouse Bone morphogenetic protein 4, BMP-4 ELISA Kit

Cat. Num. AAA15551

• Gene Names: Bmp4; Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• Reactivity: Mouse
• Synonyms: bone morphogenetic protein 4; N/A; Mouse Bone morphogenetic protein 4, BMP-4 ELISA Kit; Mouse Bone morphogenetic protein 4; BMP-4 ELISA Kit; BMP2B; BMP2B1; MCOPS6; OFC11; ZYME; bone morphogenetic protein 2B; BMP4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse BMP-4. No significant cross-reactivity or interference between mouse BMP-4 and analogues was observed.
• Sequence Length: 408
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma and tissue homogenates
• Detection Range: 0.625 pg/ml-40 pg/ml
• Sensitivity: < 0.156 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for BMP4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

References

Ah Receptor Activation by Dioxin Disrupts Activin, BMP, and WNT Signals During the Early Differentiation of Mouse Embryonic Stem Cells and Inhibits Cardiomyocyte Functions. Qin Wang, Hisaka Kurita, Vinicius Carreira, Chia-I Ko, Yunxia Fan, Xiang Zhang, Jacek Biesiada, Mario Medvedovic and Alvaro Puga (2015)

NCBI and Uniprot Product Information

• NCBI GI #: 121949823
• NCBI GeneID: 12159
• NCBI Accession #: NP_031580.2
• NCBI GenBank Nucleotide #: NM_007554.2
• UniProt Accession #: P21275
• Molecular Weight: 46,497 Da
• NCBI Official Full Name: bone morphogenetic protein 4
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: Bmp4
• NCBI Official Synonym Symbols: Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• NCBI Protein Information: bone morphogenetic protein 4; bone morphogenetic protein 2B
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: Bmp4
• UniProt Synonym Gene Names: Bmp-4; Dvr-4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_MOUSE