Rat Heparin-binding Epidermal Growth Factor like-Growth Factor ELISA Kit | HB-EGF elisa kit

Rat Heparin-binding Epidermal Growth Factor like-Growth Factor ELISA Kit

Cat. Num. AAA16105

• Gene Names: HBEGF; HB-EGF
• Reactivity: Rat
• Synonyms: Heparin-binding Epidermal Growth Factor like-Growth Factor; N/A; Rat Heparin-binding Epidermal Growth Factor like-Growth Factor ELISA Kit; HB-EGF elisa kit

• Reactivity: Rat
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/ml

• Intended Uses: This HBEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HBEGF. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for HB-EGF elisa kit

Principle of the assay: HBEGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBEGF antibody and an HBEGF-HRP conjugate. The assay sample and buffer are incubated together with HBEGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBEGF concentration since HBEGF from samples and HBEGF-HRP conjugate compete for the anti-HBEGF antibody binding site. Since the number of sites is limited, as more sites are occupied by HBEGF from the sample, fewer sites are left to bind HBEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBEGF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HB-EGF elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4761593
• NCBI GeneID: 395654
• Molecular Weight: 22,541 Da
• NCBI Official Full Name: heparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: HB-EGF
• NCBI Protein Information: proheparin-binding EGF-like growth factor
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: HEGFL; HB-EGF; HBEGF
• UniProt Entry Name: HBEGF_CHICK