Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit | EGFR elisa kit
Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Synonyms
Epidermal Growth Factor Receptor (EGFR); N/A; Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit; ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of EGFR. No significant cross-reactivity or interference between EGFR and analogues was observed.
Sequence Length
1167
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
1.21ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGFR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGFR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for EGFR elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EGFR in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EGFR. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometr ically at a wavelength of 450nm +/- 10nm. The concentration of EGFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EGFR. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometr ically at a wavelength of 450nm +/- 10nm. The concentration of EGFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for EGFR elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
69,228 Da
NCBI Official Full Name
epidermal growth factor receptor
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
