Rabbit Cyclooxygenase 2 ELISA Kit | COX2 elisa kit
Rabbit Cyclooxygenase 2 ELISA Kit
Gene Names
PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
Reactivity
Rabbit
Synonyms
Cyclooxygenase 2; N/A; Rabbit Cyclooxygenase 2 ELISA Kit; COX2 elisa kit
Reactivity
Rabbit
Specificity
This assay has high sensitivity and excellent specificity for detection of COX-2. No significant cross-reactivity or interference between COX-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COX-2 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for COX2 elisa kit
Principle of the Assay: COX-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COX-2 antibody and an COX-2-HRP conjugate. The assay sample and buffer are incubated together with COX-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX-2 concentration since COX-2 from samples and COX-2-HRP conjugate compete for the anti-COX-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by COX-2 from the sample, fewer sites are left to bind COX-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX-2 concentration in each sample is interpolated from this standard curve.
Intended Uses: This COX-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit COX-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This COX-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit COX-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for COX2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,996 Da
NCBI Official Full Name
cyclooxygenase-2
NCBI Official Synonym Full Names
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
NCBI Official Symbol
PTGS2
NCBI Official Synonym Symbols
COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
NCBI Protein Information
prostaglandin G/H synthase 2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2
UniProt Protein Name
Prostaglandin G/H synthase 2
UniProt Gene Name
PTGS2
UniProt Synonym Gene Names
COX2; COX-2; PGH synthase 2; PGHS-2
UniProt Entry Name
PGH2_HUMAN
