Rat Aldehyde Dehydrogenase ELISA Kit | ALDH elisa kit

Rat Aldehyde Dehydrogenase ELISA Kit

Cat. Num. AAA16203

• Gene Names: Aldh; 146084_at; ACDH; ALDH; ALDHA1; CG 3752; CG3752; DmelCG3752
• Reactivity: Rat
• Synonyms: Aldehyde Dehydrogenase; N/A; Rat Aldehyde Dehydrogenase ELISA Kit; ALDH elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ALDH. No significant cross-reactivity or interference between ALDH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALDH and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ALDH elisa kit

Principle of the Assay: ALDH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALDH antibody and an ALDH-HRP conjugate. The assay sample and buffer are incubated together with ALDH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH concentration since ALDH from samples and ALDH-HRP conjugate compete for the anti-ALDH antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH from the sample, fewer sites are left to bind ALDH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH concentration in each sample is interpolated from this standard curve.

Intended Uses: This ALDH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ALDH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for ALDH elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 20129399
• NCBI GeneID: 34256
• NCBI Accession #: NP_609285.1
• NCBI GenBank Nucleotide #: NM_135441.2
• Molecular Weight: 57,019 Da
• NCBI Official Full Name: aldehyde dehydrogenase, isoform A
• NCBI Official Synonym Full Names: Aldehyde dehydrogenase
• NCBI Official Symbol: Aldh
• NCBI Official Synonym Symbols: 146084_at; ACDH; ALDH; ALDHA1; CG 3752; CG3752; DmelCG3752
• NCBI Protein Information: CG3752 gene product from transcript CG3752-RA; Aldh-PA; Aldh-PB; CG3752-PA; CG3752-PB; acetaldehyde dehydrogenase
• UniProt Protein Name: Aldehyde dehydrogenase
• UniProt Gene Name: Aldh
• UniProt Synonym Gene Names: CG3752; Dmel_CG3752
• UniProt Entry Name: Q9VLC5_DROME