Mouse Ovalbumin specific Immunoglobulin G1 ELISA Kit | OVA sIgG1 elisa kit
Mouse Ovalbumin specific Immunoglobulin G1 ELISA Kit
Reactivity
Mouse
Synonyms
Ovalbumin specific Immunoglobulin G1; N/A; Mouse Ovalbumin specific Immunoglobulin G1 ELISA Kit; OVA sIgG1 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 mug/mL.
Intended Uses
This OVA-sIgG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OVA-sIgG1. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for OVA sIgG1 elisa kit
Principle of the assay: OVA-sIgG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OVA-sIgG1 antibody and an OVA-sIgG1-HRP conjugate. The assay sample and buffer are incubated together with OVA-sIgG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OVA-sIgG1 concentration since OVA-sIgG1 from samples and OVA-sIgG1-HRP conjugate compete for the anti-OVA-sIgG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OVA-sIgG1 from the sample, fewer sites are left to bind OVA-sIgG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OVA-sIgG1 concentration in each sample is interpolated from this standard curve.
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