Mouse Procollagen C Proteinase Enhancer 1 ELISA Kit | PCPE1 elisa kit

Mouse Procollagen C Proteinase Enhancer 1 ELISA Kit

Cat. Num. AAA16224

• Gene Names: PCOLCE; PCPE; PCPE1; PCPE-1
• Reactivity: Mouse
• Synonyms: Procollagen C Proteinase Enhancer 1; N/A; Mouse Procollagen C Proteinase Enhancer 1 ELISA Kit; PCPE1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PCPE1. No significant cross-reactivity or interference between PCPE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCPE1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PCPE1 elisa kit

Principle of the Assay: PCPE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCPE1 antibody and an PCPE1-HRP conjugate. The assay sample and buffer are incubated together with PCPE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCPE1 concentration since PCPE1 from samples and PCPE1-HRP conjugate compete for the anti-PCPE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PCPE1 from the sample, fewer sites are left to bind PCPE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCPE1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PCPE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PCPE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for PCPE1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 157653329
• NCBI GeneID: 5118
• NCBI Accession #: NP_002584.2
• NCBI GenBank Nucleotide #: NM_002593.3
• Molecular Weight: 47,972 Da
• NCBI Official Full Name: procollagen C-endopeptidase enhancer 1
• NCBI Official Synonym Full Names: procollagen C-endopeptidase enhancer
• NCBI Official Symbol: PCOLCE
• NCBI Official Synonym Symbols: PCPE; PCPE1; PCPE-1
• NCBI Protein Information: procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen, type 1, COOH-terminal proteinase enhancer
• UniProt Protein Name: Procollagen C-endopeptidase enhancer 1
• UniProt Gene Name: PCOLCE
• UniProt Synonym Gene Names: PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1
• UniProt Entry Name: PCOC1_HUMAN