Human cleaved microtubule-associated protein tau ELISA Kit | C-MAPT/C-TAU elisa kit
Human cleaved microtubule-associated protein tau, C-MAPT/C-TAU ELISA Kit
Reactivity
Human
Synonyms
cleaved microtubule-associated protein tau; N/A; Human cleaved microtubule-associated protein tau, C-MAPT/C-TAU ELISA Kit; Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit; cleaved form of DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MSTD; MTBT1; MTBT2; PPND; TAU; G protein beta1/gamma2 subunit-interacting factor 1; microtubule-associated protein tau; isoform 4; C-MAPT/C-TAU elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human C-MAPT/C-TAU. No significant cross-reactivity or interference between human C-MAPT/C-TAU and analogues was observed.
Samples
Serum, plasma, cerebrospinal fluid (csf), tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 15.6 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for C-MAPT/C-TAU elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C-MAPT/C-TAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C-MAPT/C-TAU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C-MAPT/C-TAU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C-MAPT/C-TAU bound in the initial step. The color development is stopped and the intensity of the color is measured.
