Rabbit Interleukin 1beta ELISA Kit | IL-1beta elisa kit
Rabbit Interleukin 1beta ELISA Kit
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Rabbit
Synonyms
Interleukin 1beta; N/A; Rabbit Interleukin 1beta ELISA Kit; Rabbit Interleukin 1b ELISA Kit; IL-1beta elisa kit
Reactivity
Rabbit
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for IL-1beta elisa kit
Intended Uses: This IL-1beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IL-1beta. This ELISA kit for research use only!
Principle of the Assay: IL-1beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-1beta antibody and an IL-1beta-HRP conjugate. The assay sample and buffer are incubated together with IL-1beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1beta concentration since IL-1beta from samples and IL-1beta-HRP conjugate compete for the anti-IL-1beta antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1beta from the sample, fewer sites are left to bind IL-1beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1beta concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IL-1beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-1beta antibody and an IL-1beta-HRP conjugate. The assay sample and buffer are incubated together with IL-1beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1beta concentration since IL-1beta from samples and IL-1beta-HRP conjugate compete for the anti-IL-1beta antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1beta from the sample, fewer sites are left to bind IL-1beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1beta concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-1beta elisa kit
References
Solvent/Detergent Virally Inactivated Serum Eye Drops Restore Healthy Ocular Epithelium in a Rabbit Model of Dry-Eye Syndrome. Ching-Li Tseng, Zhi-Yu Chen, Ting-Yi Renn, Shun-Hung Hsiao, Thierry Burnouf (2016)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,748 Da
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1, beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
NCBI Protein Information
interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
UniProt Entry Name
IL1B_HUMAN
