Fish Interleukin 1beta (IL- 1beta) ELISA Kit | IL- 1beta elisa kit

Fish Interleukin 1beta (IL- 1beta) ELISA Kit

Cat. Num. AAA14987

• Gene Names: IL1B; IL-1; IL1F2; IL1-BETA
• Reactivity: Fish
• Synonyms: Interleukin 1beta (IL- 1beta); N/A; Fish Interleukin 1beta (IL- 1beta) ELISA Kit; Interleukin 1b (IL- 1b); Fish Interleukin 1b (IL- 1b) ELISA Kit; IL- 1beta elisa kit

• Reactivity: Fish
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish IL- 1beta. No significant cross-reactivity or interference between fish IL- 1beta and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, tissue homogenates
• Detection Range: 3.12 pg/ml-200 pg/ml
• Sensitivity: < 0.78 pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for IL- 1beta elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL- 1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL- 1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL- 1beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL- 1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 10835145
• NCBI GeneID: 3553
• NCBI Accession #: NP_000567.1
• NCBI GenBank Nucleotide #: NM_000576.2
• UniProt Accession #: P01584; Q53X59; Q53XX2; Q7M4S7; Q7RU01; Q96HE5; Q9UCT6; O43645
• Molecular Weight: 30,748 Da
• NCBI Official Full Name: interleukin-1 beta proprotein
• NCBI Official Synonym Full Names: interleukin 1, beta
• NCBI Official Symbol: IL1B
• NCBI Official Synonym Symbols: IL-1; IL1F2; IL1-BETA
• NCBI Protein Information: interleukin-1 beta; IL-1 beta; catabolin; OTTHUMP00000162031; preinterleukin 1 beta; pro-interleukin-1-beta
• UniProt Protein Name: Interleukin-1 beta
• UniProt Gene Name: IL1B
• UniProt Synonym Gene Names: IL1F2
• UniProt Entry Name: IL1B_HUMAN