Canine Coagulation Factor VIII ELISA Kit | F8 elisa kit
Canine Coagulation Factor VIII ELISA Kit
Reactivity
Canine
Synonyms
Coagulation Factor VIII; N/A; Canine Coagulation Factor VIII ELISA Kit; F8 elisa kit
Reactivity
Canine
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Sandwich
Sensitivity
1.0pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for F8 elisa kit
Intended Uses: This FVII ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FVII. This ELISA kit for research use only!
Principle of the Assay: FVII ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FVII. Standards or samples are then added to the microtiter plate wells and FVII if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FVII present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FVII are added to each well to "sandwich" the FVII immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FVII and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FVII concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FVII ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FVII. Standards or samples are then added to the microtiter plate wells and FVII if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FVII present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FVII are added to each well to "sandwich" the FVII immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FVII and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FVII concentration in each sample is interpolated from this standard curve.
Product Categories/Family for F8 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
coagulation factor VIII
NCBI Official Symbol
F8
NCBI Protein Information
coagulation factor VIII
