Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of IFNgamma. No significant cross-reactivity or interference between IFNgamma and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNgamma and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for IFN gamma elisa kit
Intended Uses: This IFNgamma ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IFNgamma. This ELISA kit for research use only!
Principle of the Assay: IFNgamma ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IFNgamma. Standards or samples are then added to the microtiter plate wells and IFNgamma if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFNgamma present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFNgamma are added to each well to "sandwich" the IFNgamma immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFNgamma and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNgamma concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IFNgamma ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IFNgamma. Standards or samples are then added to the microtiter plate wells and IFNgamma if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFNgamma present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFNgamma are added to each well to "sandwich" the IFNgamma immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFNgamma and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNgamma concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IFN gamma elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,348 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG
NCBI Official Synonym Symbols
IFG; IFI
NCBI Protein Information
interferon gamma; IFN-gamma; immune interferon
UniProt Protein Name
Interferon gamma
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_HUMAN