Canine Interferon Gamma ELISA Kit | IFN-gamma elisa kit
Canine Interferon Gamma ELISA Kit
Gene Names
IFNG; IFG; IFI
Reactivity
Canine
Synonyms
Interferon Gamma; N/A; Canine Interferon Gamma ELISA Kit; IFN-gamma elisa kit
Reactivity
Canine
Specificity
Specifically recognize IFN-gamma, no obvious cross reaction with other analogues
Sequence Length
166
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for IFN-gamma elisa kit
Background: IFNgamma, also known as Interferon gamma or type II interferon, is a cytokine primarily produced by activated T cells and natural killer cells. It can stimulate various immune cells, such as macrophages, B cells, and NK cells, and has important immunoregulatory functions. IFNgamma can not only induce cellular antiviral responses, but also promote inflammatory reactions, enhance MHC molecule expression, induce apoptosis of tumor cells, and promote class switching of antibodies. IFNgamma plays an important role in immune responses. It can regulate the proliferation, differentiation, survival, and function of immune cells by activating various signaling pathways, and is closely related to the occurrence and development of various autoimmune diseases, infectious diseases, tumors, and other diseases.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti IFN-gamma antibody was precoated onto the 96-well plate. The biotin conjugated anti IFN-gamma antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with IFN-gamma conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IFN-gamma in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti IFN-gamma antibody was precoated onto the 96-well plate. The biotin conjugated anti IFN-gamma antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with IFN-gamma conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IFN-gamma in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,348 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG
NCBI Official Synonym Symbols
IFG; IFI
NCBI Protein Information
interferon gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_HUMAN
