Human Contactin 1 ELISA Kit | CNTN1 elisa kit

Human Contactin 1 ELISA Kit

Cat. Num. AAA16357

• Gene Names: CNTN1; F3; GP135; MYPCN
• Reactivity: Human
• Synonyms: Contactin 1; Human Contactin 1 ELISA Kit; CNTN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CNTN1. No significant cross-reactivity or interference between CNTN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNTN1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Highest Standards: 10 ng/mL
• Concentration of Standards: Concentration of Standards
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CNTN1 elisa kit

Principle of the Assay: CNTN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CNTN1. Standards or samples are then added to the microtiter plate wells and CNTN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CNTN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CNTN1 are added to each well to "sandwich" the CNTN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CNTN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNTN1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This CNTN1 ELISA kit is a 1.5-hour solid-phase ELISA designed for the quantitative determination of Human CNTN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for CNTN1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 28373119
• NCBI GeneID: 1272
• NCBI Accession #: NP_778203.1
• NCBI GenBank Nucleotide #: NM_175038.2
• Molecular Weight: 70,604 Da
• NCBI Official Full Name: contactin-1 isoform 2
• NCBI Official Synonym Full Names: contactin 1
• NCBI Official Symbol: CNTN1
• NCBI Official Synonym Symbols: F3; GP135; MYPCN
• NCBI Protein Information: contactin-1; glycoprotein gP135; neural cell surface protein F3
• UniProt Protein Name: Contactin-1
• UniProt Gene Name: CNTN1
• UniProt Entry Name: CNTN1_HUMAN