Human Augmenter of Liver Regeneration ELISA Kit | ALR elisa kit
Human Augmenter of Liver Regeneration ELISA Kit
Gene Names
KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
Reactivity
Human
Synonyms
Augmenter of Liver Regeneration; N/A; Human Augmenter of Liver Regeneration ELISA Kit; ALR elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ALR. No significant cross-reactivity or interference between ALR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALR and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive or Sandwich
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for ALR elisa kit
Intended Uses: This ALR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALR. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: ALR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALR. Standards or samples are then added to the microtiter plate wells and ALR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALR present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALR are added to each well to "sandwich" the ALR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALR concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ALR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALR. Standards or samples are then added to the microtiter plate wells and ALR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALR present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALR are added to each well to "sandwich" the ALR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALR concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ALR elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
593,677 Da
NCBI Official Full Name
ALR
NCBI Official Synonym Full Names
lysine (K)-specific methyltransferase 2D
NCBI Official Symbol
KMT2D
NCBI Official Synonym Symbols
ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
NCBI Protein Information
histone-lysine N-methyltransferase 2D; ALL1-related protein; Kabuki make-up syndrome; Kabuki mental retardation syndrome; trinucleotide repeat containing 21; histone-lysine N-methyltransferase MLL2; myeloid/lymphoid or mixed-lineage leukemia 2
UniProt Protein Name
Histone-lysine N-methyltransferase 2D
UniProt Gene Name
KMT2D
UniProt Synonym Gene Names
ALR; MLL2; MLL4; Lysine N-methyltransferase 2D
UniProt Entry Name
KMT2D_HUMAN
