Human Dihydrotestosterone ELISA Kit | DHT elisa kit
Human Dihydrotestosterone ELISA Kit
Reactivity
Human
Synonyms
Dihydrotestosterone; N/A; Human Dihydrotestosterone ELISA Kit; DHT elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of DHT. No significant cross-reactivity or interference between DHT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DHT and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for DHT elisa kit
Intended Uses: This DHT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DHT. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: DHT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DHT antibody and an DHT-HRP conjugate. The assay sample and buffer are incubated together with DHT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHT concentration since DHT from samples and DHT-HRP conjugate compete for the anti-DHT antibody binding site. Since the number of sites is limited, as more sites are occupied by DHT from the sample, fewer sites are left to bind DHT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHT concentration in each sample is interpolated from this standard curve.
Principle of the Assay: DHT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DHT antibody and an DHT-HRP conjugate. The assay sample and buffer are incubated together with DHT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHT concentration since DHT from samples and DHT-HRP conjugate compete for the anti-DHT antibody binding site. Since the number of sites is limited, as more sites are occupied by DHT from the sample, fewer sites are left to bind DHT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHT concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,872 Da
NCBI Official Full Name
D-hydantoinase/dihydropyrimidinase Dht
NCBI Official Symbol
dht
NCBI Protein Information
D-hydantoinase/dihydropyrimidinase Dht
UniProt Protein Name
D-hydantoinase/dihydropyrimidinase Dht
UniProt Gene Name
dht
UniProt Synonym Gene Names
RLO149_c023310
UniProt Entry Name
F7ZBB5_ROSLO
