Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of COMP. No significant cross-reactivity or interference between COMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COMP and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for COMP elisa kit
Principle of the Assay: COMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COMP antibody and an COMP-HRP conjugate. The assay sample and buffer are incubated together with COMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COMP concentration since COMP from samples and COMP-HRP conjugate compete for the anti-COMP antibody binding site. Since the number of sites is limited, as more sites are occupied by COMP from the sample, fewer sites are left to bind COMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COMP concentration in each sample is interpolated from this standard curve.
Intended Uses: This COMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine COMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This COMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine COMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for COMP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
14,872 Da
NCBI Official Full Name
ComP
UniProt Protein Name
ComP
UniProt Gene Name
comP
UniProt Entry Name
O30583_9GAMM