Human Insulin-like Growth Factors 1 Long R3 (IGF-1 LR3) ELISA Kit

Human Insulin-like Growth Factors 1 Long R3 (IGF-1 LR3) ELISA Kit

Cat. Num. AAA16712

• Reactivity: Human
• Synonyms: Insulin-like Growth Factors 1 Long R3 (IGF-1 LR3); N/A; Human Insulin-like Growth Factors 1 Long R3 (IGF-1 LR3) ELISA Kit; Insulin-like Growth Factors 1 Long R3 (IGF-1 LR3) elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IGF1-LR3. No significant cross-reactivity or interference between IGF1-LR3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGF1-LR3 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Insulin-like Growth Factors 1 Long R3 (IGF-1 LR3) elisa kit

Principle of the Assay: IGF1-LR3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IGF1-LR3 antibody and an IGF1-LR3-HRP conjugate. The assay sample and buffer are incubated together with IGF1-LR3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF1-LR3 concentration since IGF1-LR3 from samples and IGF1-LR3-HRP conjugate compete for the anti-IGF1-LR3 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF1-LR3 from the sample, fewer sites are left to bind IGF1-LR3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF1-LR3 concentration in each sample is interpolated from this standard curve.

Intended Uses: This IGF1-LR3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IGF1-LR3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!