Guinea Pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit | MCP-1 elisa kit
Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Guinea Pig
Synonyms
Monocyte chemotactic protein 1/monocyte chemotactic and activating factor; N/A; Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit; MCP-1 elisa kit
Reactivity
Guinea Pig
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This MCP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig MCP-1. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for MCP-1 elisa kit
Principle of the assay: MCP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCP-1 antibody and an MCP-1-HRP conjugate. The assay sample and buffer are incubated together with MCP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP-1 concentration since MCP-1 from samples and MCP-1-HRP conjugate compete for the anti-MCP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP-1 from the sample, fewer sites are left to bind MCP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MCP-1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
11,025 Da
NCBI Official Full Name
MCP-1
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
CCL2
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Protein Information
C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
CCL2
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1
UniProt Entry Name
CCL2_HUMAN