Chicken P53 ELISA Kit | P53 elisa kit

Chicken P53 ELISA Kit

Cat. Num. AAA16764

• Gene Names: TP53; P53; LFS1; TRP53
• Reactivity: Chicken
• Synonyms: P53; N/A; Chicken P53 ELISA Kit; P53 elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of P53. No significant cross-reactivity or interference between P53 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between P53 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for P53 elisa kit

Principle of the Assay: P53 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-P53 antibody and an P53-HRP conjugate. The assay sample and buffer are incubated together with P53-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P53 concentration since P53 from samples and P53-HRP conjugate compete for the anti-P53 antibody binding site. Since the number of sites is limited, as more sites are occupied by P53 from the sample, fewer sites are left to bind P53-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P53 concentration in each sample is interpolated from this standard curve.

Intended Uses: This P53 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken P53. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for P53 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 23491729
• NCBI GeneID: 7157
• UniProt Accession #: P04637; Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98; Q0PKT5; Q0ZAJ9; Q0ZAK0; Q0ZAK1; Q19RW5; Q19RW6; Q1HGV0; Q1HGV1; Q1HGV2
• Molecular Weight: 43,653 Da
• NCBI Official Full Name: P53
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53; antigen NY-CO-13; phosphoprotein p53; p53 tumor suppressor; tumor suppressor TP53; transformation-related protein 53
• UniProt Protein Name: Cellular tumor antigen p53
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53
• UniProt Entry Name: P53_HUMAN