Canine Interferon beta ELISA Kit | IFN-beta elisa kit

Canine Interferon beta ELISA Kit

Cat. Num. AAA16836

• Gene Names: IFNB1; IFB; IFF; IFNB
• Reactivity: Canine
• Synonyms: Interferon beta; N/A; Canine Interferon beta ELISA Kit; Canine Interferon b ELISA Kit; IFN-beta elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of IFNbeta. No significant cross-reactivity or interference between IFNbeta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNbeta and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Reactivity Note: No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for IFN-beta elisa kit

Principle of the Assay: IFNbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IFNbeta antibody and an IFNbeta-HRP conjugate. The assay sample and buffer are incubated together with IFNbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNbeta concentration since IFNbeta from samples and IFNbeta-HRP conjugate compete for the anti-IFNbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNbeta from the sample, fewer sites are left to bind IFNbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNbeta concentration in each sample is interpolated from this standard curve.

Intended Uses: This IFNbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IFNbeta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for IFN-beta elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4504603
• NCBI GeneID: 3456
• NCBI Accession #: NP_002167.1
• NCBI GenBank Nucleotide #: NM_002176.2
• Molecular Weight: 22,294 Da
• NCBI Official Full Name: interferon beta
• NCBI Official Synonym Full Names: interferon, beta 1, fibroblast
• NCBI Official Symbol: IFNB1
• NCBI Official Synonym Symbols: IFB; IFF; IFNB
• NCBI Protein Information: interferon beta; IFN-beta; fibroblast interferon
• UniProt Protein Name: Interferon beta
• UniProt Gene Name: IFNB1
• UniProt Synonym Gene Names: IFB; IFNB; IFN-beta
• UniProt Entry Name: IFNB_HUMAN