Reactivity
Monkey
Specificity
This kit recognizes Monkey IFN-beta in samples. No significant cross-reactivity or interference between Monkey IFN-beta and analogues was observed.
Sequence Length
187
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.63-1000pg/mL
Sensitivity
9.38pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey IFN-beta were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey IFN-beta were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Related Product Information for IFN-beta elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey IFN-beta concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey IFN-beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey IFN-beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey IFN-beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey IFN-beta. You can calculate the concentration of Monkey IFN-beta in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey IFN-beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey IFN-beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey IFN-beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey IFN-beta. You can calculate the concentration of Monkey IFN-beta in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,294 Da
NCBI Official Full Name
interferon beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB
NCBI Protein Information
interferon beta; IFN-beta; fibroblast interferon
UniProt Protein Name
Interferon beta
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN