Human 5 Phosphoribosyl 1 Pyrophosphate ELISA Kit | PRPP elisa kit

Human 5 Phosphoribosyl 1 Pyrophosphate ELISA Kit

Cat. Num. AAA17172

• Reactivity: Human
• Synonyms: 5 Phosphoribosyl 1 Pyrophosphate; N/A; Human 5 Phosphoribosyl 1 Pyrophosphate ELISA Kit; PRPP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRPP. No significant cross-reactivity or interference between PRPP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRPP and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PRPP elisa kit

Principle of the Assay: PRPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PRPP antibody and an PRPP-HRP conjugate. The assay sample and buffer are incubated together with PRPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRPP concentration since PRPP from samples and PRPP-HRP conjugate compete for the anti-PRPP antibody binding site. Since the number of sites is limited, as more sites are occupied by PRPP from the sample, fewer sites are left to bind PRPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRPP concentration in each sample is interpolated from this standard curve.

Intended Uses: This PRPP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRPP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!