Standard Curve (Sample)
Human 5-lipoxygenase (5-LOX) ELISA Kit | 5-LOX elisa kit
Human 5-lipoxygenase (5-LOX) ELISA Kit
Gene Names
ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
Reactivity
Human
Synonyms
5-lipoxygenase (5-LOX); Human 5-lipoxygenase (5-LOX) ELISA Kit; 5-LOX elisa kit
Reactivity
Human
Sequence Length
50
Assay Type
Sandwich
Detection Range
10 ng/mL-0.156 ng/mL
Sensitivity
Up to 0.05 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for 5-LOX elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human 5-LOX monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56,291 Da
NCBI Official Full Name
5-lipoxygenase, partial
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase
NCBI Official Symbol
ALOX5
NCBI Official Synonym Symbols
5-LO; 5LPG; LOG5; 5-LOX
NCBI Protein Information
arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform
UniProt Protein Name
Arachidonate 5-lipoxygenase
UniProt Gene Name
ALOX5
UniProt Synonym Gene Names
LOG5; 5-LO; 5-lipoxygenase
UniProt Entry Name
LOX5_HUMAN
Similar Products
Product Notes
The Human 5-LOX alox5 (Catalog #AAA22386) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA22386 ELISA Kit recognizes Human 5-LOX. It is sometimes possible for the material contained within the vial of "5-lipoxygenase (5-LOX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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