Bovine Hydroxylysyl Pyridinoline ELISA Kit | HP elisa kit
Bovine Hydroxylysyl Pyridinoline ELISA Kit
Reactivity
Bovine
Synonyms
Hydroxylysyl Pyridinoline; N/A; Bovine Hydroxylysyl Pyridinoline ELISA Kit; HP elisa kit
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100 nmoL/L
Sensitivity
1.0 nmoL/L
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for HP elisa kit
Intended Uses: This Hy-PYD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Hy-PYD. This ELISA kit for research use only!
Principle of the Assay||Hy-PYD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Hy-PYD antibody and an Hy-PYD-HRP conjugate. The assay sample and buffer are incubated together with Hy-PYD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Hy-PYD concentration since Hy-PYD from samples and Hy-PYD-HRP conjugate compete for the anti-Hy-PYD antibody binding site. Since the number of sites is limited, as more sites are occupied by Hy-PYD from the sample, fewer sites are left to bind Hy-PYD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hy-PYD concentration in each sample is interpolated from this standard curve.
Principle of the Assay||Hy-PYD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Hy-PYD antibody and an Hy-PYD-HRP conjugate. The assay sample and buffer are incubated together with Hy-PYD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Hy-PYD concentration since Hy-PYD from samples and Hy-PYD-HRP conjugate compete for the anti-Hy-PYD antibody binding site. Since the number of sites is limited, as more sites are occupied by Hy-PYD from the sample, fewer sites are left to bind Hy-PYD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hy-PYD concentration in each sample is interpolated from this standard curve.
