Human von Willebrand factor type A, EGF And Pentraxin Domain containing protein 1 ELISA Kit | SVEP1 elisa kit
Human von Willebrand factor type A, EGF And Pentraxin Domain containing protein 1 ELISA Kit
Reactivity
Human
Synonyms
von Willebrand factor type A, EGF And Pentraxin Domain containing protein 1; N/A; Human von Willebrand factor type A, EGF And Pentraxin Domain containing protein 1 ELISA Kit; SVEP1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SVEP1. No significant cross-reactivity or interference between SVEP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SVEP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for SVEP1 elisa kit
Intended Uses: This SVEP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SVEP1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SVEP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SVEP1 antibody and an SVEP1-HRP conjugate. The assay sample and buffer are incubated together with SVEP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SVEP1 concentration since SVEP1 from samples and SVEP1-HRP conjugate compete for the anti-SVEP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SVEP1 from the sample, fewer sites are left to bind SVEP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SVEP1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SVEP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SVEP1 antibody and an SVEP1-HRP conjugate. The assay sample and buffer are incubated together with SVEP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SVEP1 concentration since SVEP1 from samples and SVEP1-HRP conjugate compete for the anti-SVEP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SVEP1 from the sample, fewer sites are left to bind SVEP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SVEP1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1
