Standard Curve (Sample)
Canine Von Willebrand Factor (vWF) ELISA Kit | vWF elisa kit
Canine Von Willebrand Factor (vWF) ELISA Kit
Gene Names
VWF; VWD; F8VWF
Reactivity
Canine
Synonyms
Von Willebrand Factor (vWF); N/A; Canine Von Willebrand Factor (vWF) ELISA Kit; F8VWF; VWD; von Willebrand antigen 2; vWF elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of vWF. No significant cross-reactivity or interference between vWF and analogues was observed.
Sequence Length
351
Assay Type
Quantitative Competitive
Samples
Canine plasma
Detection Range
9.88-800ng/mL
Sensitivity
< 3.84ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level vWF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level vWF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for vWF elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to vWF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled vWF and unlabeled vWF (Standards or samples) with the pre-coated antibody specific to vWF. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of vWF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of vWF in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of vWF in canine plasma.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of vWF in canine plasma.
Product Categories/Family for vWF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,745 Da
NCBI Official Full Name
von Willebrand factor preproprotein
NCBI Official Synonym Full Names
von Willebrand factor
NCBI Official Symbol
VWF
NCBI Official Synonym Symbols
VWD; F8VWF
NCBI Protein Information
von Willebrand factor
UniProt Protein Name
von Willebrand factor
UniProt Gene Name
VWF
UniProt Synonym Gene Names
F8VWF; vWF
UniProt Entry Name
VWF_HUMAN
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Product Notes
The Canine vWF vwf (Catalog #AAA20619) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA20619 ELISA Kit recognizes Canine vWF. It is sometimes possible for the material contained within the vial of "Von Willebrand Factor (vWF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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