Canine Von Willebrand Factor (vWF) ELISA Kit | vWF elisa kit
Canine Von Willebrand Factor (vWF) ELISA Kit
Gene Names
VWF; VWD; F8VWF
Reactivity
Canine
Synonyms
Von Willebrand Factor (vWF); N/A; Canine Von Willebrand Factor (vWF) ELISA Kit; F8VWF; VWD; von Willebrand antigen 2; vWF elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of vWF. No significant cross-reactivity or interference between vWF and analogues was observed.
Sequence Length
351
Assay Type
Quantitative Competitive
Samples
Canine plasma
Detection Range
9.88-800ng/mL
Sensitivity
< 3.84ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level vWF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level vWF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for vWF elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to vWF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled vWF and unlabeled vWF (Standards or samples) with the pre-coated antibody specific to vWF. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of vWF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of vWF in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of vWF in canine plasma.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of vWF in canine plasma.
Product Categories/Family for vWF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,745 Da
NCBI Official Full Name
von Willebrand factor preproprotein
NCBI Official Synonym Full Names
von Willebrand factor
NCBI Official Symbol
VWF
NCBI Official Synonym Symbols
VWD; F8VWF
NCBI Protein Information
von Willebrand factor
UniProt Protein Name
von Willebrand factor
UniProt Gene Name
VWF
UniProt Synonym Gene Names
F8VWF; vWF
UniProt Entry Name
VWF_HUMAN
