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Standard Curve (Sample)

Chicken Alkaline Phosphatase, Intestinal ELISA Kit | ALPI elisa kit

Chicken Alkaline Phosphatase, Intestinal ELISA Kit

Gene Names
ALPI; IAP
Reactivity
Chicken
Synonyms
Alkaline Phosphatase, Intestinal; N/A; Chicken Alkaline Phosphatase, Intestinal ELISA Kit; ALPI elisa kit
Ordering
 
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For Research Use Only!
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of iALP. No significant cross-reactivity or interference between iALP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iALP and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ALPI elisa kit
Principle of the Assay: iALP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-iALP antibody and an iALP-HRP conjugate. The assay sample and buffer are incubated together with iALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iALP concentration since iALP from samples and iALP-HRP conjugate compete for the anti-iALP antibody binding site. Since the number of sites is limited, as more sites are occupied by iALP from the sample, fewer sites are left to bind iALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iALP concentration in each sample is interpolated from this standard curve.

Intended Uses: This iALP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken iALP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for ALPI elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
248
Molecular Weight
56,812 Da
NCBI Official Full Name
Alkaline phosphatase, intestinal
NCBI Official Synonym Full Names
alkaline phosphatase, intestinal
NCBI Official Symbol
ALPI
NCBI Official Synonym Symbols
IAP
NCBI Protein Information
intestinal-type alkaline phosphatase; Kasahara isozyme; glycerophosphatase; alkaline phosphomonoesterase; intestinal alkaline phosphatase
UniProt Protein Name
Intestinal-type alkaline phosphatase
UniProt Gene Name
ALPI
UniProt Synonym Gene Names
IAP; Intestinal alkaline phosphatase
UniProt Entry Name
PPBI_HUMAN

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Product Notes

The Chicken ALPI alpi (Catalog #AAA17236) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17236 ELISA Kit recognizes Chicken ALPI. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Intestinal, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.