Human Peptidyl Arginine Deiminase Type IV ELISA Kit | PADI4 elisa kit

Human Peptidyl Arginine Deiminase Type IV ELISA Kit

Cat. Num. AAA17407

• Gene Names: PADI4; PAD; PAD4; PDI4; PDI5; PADI5
• Reactivity: Human
• Synonyms: Peptidyl Arginine Deiminase Type IV; N/A; Human Peptidyl Arginine Deiminase Type IV ELISA Kit; PADI4/PADPAD4/PDI4/PDI5EC 3.5.3.15/peptidyl arginine deiminase; type V/Peptidylarginine deiminase IV/Protein-arginine deiminase type IV/protein-arginine deiminase type-4; PADI4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PADI4. No significant cross-reactivity or interference between PADI4 and analogues was observed.
• Sequence Length: 105
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 0.188-12ng/ml
• Sensitivity: 0.113ng/ml

• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for PADI4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 50299840
• NCBI GeneID: 23569
• UniProt Accession #: Q6EVJ7; Q9UM07
• Molecular Weight: 10,961 Da
• NCBI Official Full Name: peptidyl arginine deiminase type IV, partial
• NCBI Official Synonym Full Names: peptidyl arginine deiminase, type IV
• NCBI Official Symbol: PADI4
• NCBI Official Synonym Symbols: PAD; PAD4; PDI4; PDI5; PADI5
• NCBI Protein Information: protein-arginine deiminase type-4
• UniProt Protein Name: Peptidyl arginine deiminase type IV
• UniProt Gene Name: PADI4
• UniProt Entry Name: Q6EVJ7_HUMAN