Mouse COX IV Monoclonal Antibody | anti-COXIV antibody

COX IV Mouse mAb

Cat. Num. AAA30792

• Gene Names: COX4I1; COX4; COXIV; COX4-1
• Reactivity: Human, Mouse, Rat, Hamster, Goat, Monkey
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Synonyms: COX IV, Antibody; COX IV Mouse mAb; AL024441; COX 4; COX IV 1; COX IV; COX IV-1; Cox4; COX41_HUMAN; Cox4a; COX4B; COX4I1; COX4I2; COX4L2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome C Oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); Cytochrome c oxydase subunit; dJ857M17.2; MGC105470; MGC72016.; anti-COXIV antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Hamster, Goat, Monkey
• Clonality: Monoclonal
• Specificity: This antibody detects endogenous levels of COX IV and does not cross-react with related proteins.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-COXIV antibody: Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000; ICC: 1:150; FC/FACS/FCM: 1:100
• Immunogen: A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

FCM (Flow Cytometry)

(Flow Cytometry analysis of K562 cells stained with COX4 (red. 1/100 dilution). followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control. normal mouse IgG.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8.1:50 diluted).showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).)

ICC (Immunocytochemistry)

(Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.)

IP (Immunoprecipitation)

(Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.)

WB (Western Blot)

(Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.)

WB (Western Blot)

(Western blot detection of COX IV in Goat muscle.CHO-k1.COS7.3T3.Hela.C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.)

Related Product Information for anti-COXIV antibody

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes

Product Categories/Family for anti-COXIV antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502981
• NCBI GeneID: 1327
• NCBI Accession #: NP_001852.1
• NCBI GenBank Nucleotide #: NM_001861.3
• UniProt Accession #: P13073; Q6P666; B2R4J2; D3DUM7
• Molecular Weight: 19,577 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit IV isoform 1
• NCBI Official Symbol: COX4I1
• NCBI Official Synonym Symbols: COX4; COXIV; COX4-1
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX IV-1; cytochrome c oxidase polypeptide IV
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UniProt Gene Name: COX4I1
• UniProt Synonym Gene Names: COX4; COX IV-1
• UniProt Entry Name: COX41_HUMAN