Human Mammaglobin-A ELISA Kit | SCGB2A2 elisa kit
Human Mammaglobin-A ELISA Kit
Gene Names
SCGB2A2; MGB1; UGB2
Reactivity
Human
Synonyms
Mammaglobin-A; N/A; Human Mammaglobin-A ELISA Kit; SCGB2A2/MGB1/UGB2/Mammaglobin-1/Secretoglobin family 2A member 2; SCGB2A2 elisa kit
Reactivity
Human
Specificity
Specifically recognize SCGB2A2, no obvious cross reaction with other analogues
Sequence Length
93
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for SCGB2A2 elisa kit
Background: Mammaglobin A is a protein that belongs to the secretoglobin superfamily. It has highly specific expression in cells from most breast cancers and may be used to detect circulating or disseminated tumor cells. Mammaglobin A has become one of the most specific markers for breast cancer and for the detection of CTC and micrometastasis.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti SCGB2A2 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti SCGB2A2 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with SCGB2A2 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of SCGB2A2 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti SCGB2A2 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti SCGB2A2 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with SCGB2A2 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of SCGB2A2 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,122 Da
NCBI Official Full Name
mammaglobin-A
NCBI Official Synonym Full Names
secretoglobin family 2A member 2
NCBI Official Symbol
SCGB2A2
NCBI Official Synonym Symbols
MGB1; UGB2
NCBI Protein Information
mammaglobin-A
UniProt Protein Name
Mammaglobin-A
UniProt Gene Name
SCGB2A2
UniProt Synonym Gene Names
MGB1; UGB2
UniProt Entry Name
SG2A2_HUMAN
