Human Arginase-1 ELISA Kit | ARG1 elisa kit
Human Arginase-1 ELISA Kit
Gene Names
Arg1; arginase-1
Reactivity
Human
Synonyms
Arginase-1; N/A; Human Arginase-1 ELISA Kit; ARG1 (Arginase-1)/Liver Arginase/Liver-type arginase/Type I arginase; ARG1 elisa kit
Reactivity
Human
Specificity
Specifically recognize ARG1, no obvious cross reaction with other analogues
Sequence Length
323
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
3.125-200ng/ml
Sensitivity
1.875ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for ARG1 elisa kit
Background: Arginase 1 (ARG1) is a 35-40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ARG1 antibody was precoated onto the 96-well plate. The biotin conjugated anti ARG1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ARG1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ARG1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ARG1 antibody was precoated onto the 96-well plate. The biotin conjugated anti ARG1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ARG1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ARG1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
arginase-1
NCBI Official Symbol
Arg1
NCBI Official Synonym Symbols
arginase-1
NCBI Protein Information
arginase-1
