Human Pendrin ELISA Kit | SLC26A4 elisa kit
Human Pendrin ELISA Kit
Gene Names
SLC26A4; EVA; PDS; DFNB4; TDH2B
Reactivity
Human
Synonyms
Pendrin; N/A; Human Pendrin ELISA Kit; SLC26A4/Pendrin/PDS/Sodium-independent chloride/iodide transporter/Solute carrier family 26 member 4; SLC26A4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Slc25a5. No significant cross-reactivity or interference between Slc25a5 and analogues was observed.
Sequence Length
780
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slc25a5 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slc25a5 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for SLC26A4 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Slc25a5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Slc25a5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Slc25a5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Slc25a5 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,267 Da
NCBI Official Full Name
pendrin
NCBI Official Synonym Full Names
solute carrier family 26 member 4
NCBI Official Symbol
SLC26A4
NCBI Official Synonym Symbols
EVA; PDS; DFNB4; TDH2B
NCBI Protein Information
pendrin
UniProt Protein Name
Pendrin
UniProt Gene Name
SLC26A4
UniProt Synonym Gene Names
PDS
UniProt Entry Name
S26A4_HUMAN
