Rat Exendin 4 ELISA Kit | EXENDIN-4 elisa kit
Rat Exendin 4 ELISA Kit
Reactivity
Rat
Synonyms
Exendin 4; N/A; Rat Exendin 4 ELISA Kit; EXENDIN-4 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of EXEN4. No significant cross-reactivity or interference between EXEN4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EXEN4 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for EXENDIN-4 elisa kit
Principle of the Assay: EXEN4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-EXEN4 antibody and an EXEN4-HRP conjugate. The assay sample and buffer are incubated together with EXEN4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EXEN4 concentration since EXEN4 from samples and EXEN4-HRP conjugate compete for the anti-EXEN4 antibody binding site. Since the number of sites is limited, as more sites are occupied by EXEN4 from the sample, fewer sites are left to bind EXEN4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EXEN4 concentration in each sample is interpolated from this standard curve.
Intended Uses: This EXEN4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EXEN4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This EXEN4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EXEN4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for EXENDIN-4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
exendin-4
