Human Complement component C7 ELISA Kit | C7 elisa kit
Human Complement component C7 ELISA Kit
Reactivity
Human
Synonyms
Complement component C7; N/A; Human Complement component C7 ELISA Kit; C7 (Complement component C7); C7 elisa kit
Reactivity
Human
Specificity
Specifically recognize C7, no obvious cross reaction with other analogues
Sequence Length
843
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for C7 elisa kit
Background: Complement component 7 (C7) is a 93-kDa serum glycoprotein encoded by the C7 gene. C7 interacts with other terminal complement components (C5b, C6, C8, and C9) to form a membrane attack complex (MAC), which functions as the cytolytic effector unit of the complement system. Diseases associated with C7 include Complement Component 7 Deficiency and Immunodeficiency Due To A Late Component Of Complement Deficiency.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti C7 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti C7 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with C7 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C7 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti C7 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti C7 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with C7 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of C7 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,518 Da
NCBI Official Full Name
complement component C7
NCBI Official Synonym Full Names
complement component 7
NCBI Official Symbol
C7
NCBI Protein Information
complement component C7
UniProt Protein Name
Complement component C7
UniProt Gene Name
C7
UniProt Entry Name
CO7_HUMAN
