Monkey Complement Component 5 ELISA Kit | C5 elisa kit

Monkey Complement Component 5 ELISA Kit

Cat. Num. AAA16957

• Gene Names: C5; C5D; C5a; C5b; ECLZB; CPAMD4
• Reactivity: Monkey
• Synonyms: Complement Component 5; N/A; Monkey Complement Component 5 ELISA Kit; C5 elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of C5. No significant cross-reactivity or interference between C5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C5 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Intended Uses: This C5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for C5 elisa kit

Principle of the Assay: C5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C5. Standards or samples are then added to the microtiter plate wells and C5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C5 are added to each well to "sandwich" the C5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5 concentration in each sample is interpolated from this standard curve.

Intended Uses: This C5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for C5 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 109731812
• NCBI GeneID: 727
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: Complement component 5
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5D; C5a; C5b; ECLZB; CPAMD4
• NCBI Protein Information: complement C5; prepro-C5; C5a anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• UniProt Protein Name: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN