Reactivity
Mouse
Specificity
Specifically recognize CTNI, no obvious cross reaction with other analogues
Sequence Length
189
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
7.813-500pg/ml
Sensitivity
4.688pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Related Product Information for CTNI elisa kit
Background: cTn is made up of subunits of three different genes: cardiac troponin T (cTnT), cardiac troponin I (cTn I), and troponin C (TnC). cTn exists in cardiomyocytes in the form of CTNI-C-T complex and free cTnI, which can be further decomposed into CTNI-C complex and free cTnI after being released into the blood circulation during myocardial injury. Therefore, in addition to cTnI C-T and free cTnI, there is also CTNI-C in the blood circulation, and CTNI-C is its main form in the blood. Its metabolites are excreted from the body by the kidneys.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CTNI antibody was precoated onto the 96-well plate. The biotin conjugated anti CTNI antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CTNI conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CTNI in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CTNI antibody was precoated onto the 96-well plate. The biotin conjugated anti CTNI antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CTNI conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CTNI in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cardiac troponin I
NCBI Official Symbol
LOC100462680
NCBI Protein Information
cardiac troponin I