Standard Curve (Sample)
Mouse CTNI (Cardiac Troponin-I) ELISA Kit | CTNI elisa kit
Mouse CTNI (Cardiac Troponin-I)
Reactivity
Mouse
Synonyms
CTNI (Cardiac Troponin-I); Mouse CTNI (Cardiac Troponin-I); CTNI elisa kit
Reactivity
Mouse
Specificity
Specifically recognize CTNI, no obvious cross reaction with other analogues
Sequence Length
189
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
7.813-500pg/ml
Sensitivity
4.688pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CTNI elisa kit
Background: cTn is made up of subunits of three different genes: cardiac troponin T (cTnT), cardiac troponin I (cTn I), and troponin C (TnC). cTn exists in cardiomyocytes in the form of CTNI-C-T complex and free cTnI, which can be further decomposed into CTNI-C complex and free cTnI after being released into the blood circulation during myocardial injury. Therefore, in addition to cTnI C-T and free cTnI, there is also CTNI-C in the blood circulation, and CTNI-C is its main form in the blood. Its metabolites are excreted from the body by the kidneys.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CTNI antibody was precoated onto the 96-well plate. The biotin conjugated anti CTNI antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CTNI conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CTNI in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CTNI antibody was precoated onto the 96-well plate. The biotin conjugated anti CTNI antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CTNI conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CTNI in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cardiac troponin I
NCBI Official Symbol
LOC100462680
NCBI Protein Information
cardiac troponin I
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Product Notes
The Mouse CTNI (Catalog #AAA17770) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17770 ELISA Kit recognizes Mouse CTNI. It is sometimes possible for the material contained within the vial of "CTNI (Cardiac Troponin-I), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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