Rat T (Testosterone) ELISA Kit
Rat T (Testosterone)
Reactivity
Rat
Synonyms
T (Testosterone); N/A; Rat T (Testosterone); T (Testosterone) elisa kit
Reactivity
Rat
Specificity
Specifically recognize Testosterone, no obvious cross reaction with other analogues
Assay Type
Competitive
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for T (Testosterone) elisa kit
Background: Testosterone is the principle sex hormone responsible for the development of reproductive function in male vertebrates. Testosterone plays an important role in maintaining sex drive, sperm production, muscle strength and bone mass. A healthy level of testosterone is also protective against bone disorders such as osteoporosis.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Testosterone. During the reaction, Testosterone in the sample or standard competes with a fixed amount of Testosterone on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Testosterone. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Testosterone in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Testosterone. During the reaction, Testosterone in the sample or standard competes with a fixed amount of Testosterone on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Testosterone. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Testosterone in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
