Mouse Nuclear Antigen Monoclonal Antibody

Nuclear Antigen (Pan-Nuclear Marker) Mouse Monoclonal Antibody

Cat. Num. AAA13844

• Reactivity: Human, Mouse, Rat.; Does not react with Pig
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Synonyms: Nuclear Antigen; Monoclonal Antibody; Nuclear Antigen (Pan-Nuclear Marker) Mouse Monoclonal Antibody; anti-Nuclear Antigen antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat.; Does not react with Pig
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: NM106
• Specificity: This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 243

• Applicable Applications for anti-Nuclear Antigen antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Flow Cytometry (1-2ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-paraffin) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nuclei
• Immunogen: Nuclei of HL60 cells
• Positive Control: All human cells. Tonsil
• Preparation and Storage: Store at 2 to 8 degree C. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescentstaining of PFA-fixedHeLa cells with Pan-Nuclear Antigen Monoclonal Antibody (AAA13844) followed by goat anti-mouse IgG-CF488 (green). Membranes labeled with phalloidin (red).)

SDS-PAGE

(SDS-PAGE Analysis Purified Pan-Nuclear Antigen Monoclonal Antibody (AAA13844). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Lung stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Colon stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)

References

Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseas

NCBI and Uniprot Product Information

• NCBI GI #: 149391483
• NCBI Official Full Name: nuclear antigen, partial
• UniProt Protein Name: Nuclear antigen
• UniProt Entry Name: A6N0I9_ORYSI