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WB (Western Blot)

anti-Human, Mouse ATF-4 Antibody | anti-ATF-4 antibody

ATF-4 (Phospho-Ser219) Antibody

Gene Names
ATF4; CREB2; TXREB; CREB-2; TAXREB67
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
ATF-4; N/A; ATF-4 (Phospho-Ser219) Antibody; Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB; anti-ATF-4 antibody
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For Research Use Only!
Reactivity
Human, Mouse
Specificity
ATF-4 (Phospho-Ser219) Antibody detects endogenous levels of ATF4 only when phosphorylated at serine 219.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
351
Applicable Applications for anti-ATF-4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of serine 219.
All Sites
Human: Ser219
Mouse: Ser218
Preparation and Storage
Store at-20 degree C for 1 year.

WB (Western Blot)

(Western blot analysis of extracts from COLO205 cells, using ATF-4 (Phospho-Ser219) Antibody.)

Product Categories/Family for anti-ATF-4 antibody
References
Tsujimoto A., J. Virol. 65:1420-1426(1991). Karpinski B.A., Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992). Karpinski B.A., Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
468
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,590 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-4
NCBI Official Synonym Full Names
activating transcription factor 4
NCBI Official Symbol
ATF4
NCBI Official Synonym Symbols
CREB2; TXREB; CREB-2; TAXREB67
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-4
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-4
UniProt Gene Name
ATF4
UniProt Synonym Gene Names
CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67

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Product Notes

The ATF-4 atf4 (Catalog #AAA17966) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ATF-4 (Phospho-Ser219) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATF-4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the ATF-4 atf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF-4, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.